Abstract
In eukaryotes, nuclear proteins that are transported into nuclei have nuclear localization signals (NLSs), which are recognized by proteins called importin alpha. We isolated a rice cDNA, #61L, and the corresponding gene that encodes a protein, which shows significant homology to the importin alpha. Although the encoded protein had only 23-27% amino acid identity to the importin alphas from various organisms including plants, the fusion protein with glutathione S-transferase showed a specific binding activity to the NLS of SV40 T-antigen. These results suggest that the rice #61L protein is a novel importin alpha in plants.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Antigens, Viral, Tumor / chemistry
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Antigens, Viral, Tumor / metabolism
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Arabidopsis / metabolism
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Binding Sites
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Cell Nucleus / physiology
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Genes, Plant
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Glutathione Transferase / biosynthesis
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Humans
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Molecular Sequence Data
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Nuclear Proteins / chemistry
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Nuclear Proteins / genetics*
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Nuclear Proteins / metabolism*
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Open Reading Frames
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Oryza / genetics*
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Oryza / metabolism*
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Phylogeny*
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Recombinant Fusion Proteins / biosynthesis
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Recombinant Fusion Proteins / chemistry
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Recombinant Fusion Proteins / metabolism
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Saccharomyces cerevisiae / metabolism
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Sequence Alignment
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Sequence Homology, Amino Acid
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Simian virus 40 / metabolism
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Xenopus
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alpha Karyopherins
Substances
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Antigens, Viral, Tumor
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Nuclear Proteins
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Recombinant Fusion Proteins
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alpha Karyopherins
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Glutathione Transferase
Associated data
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GENBANK/AB006788
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GENBANK/D78504