Abstract
The regulation of murein hydrolases is a critical aspect of peptidoglycan growth and metabolism. In the present study, we demonstrate that mutations within the Staphylococcus aureus virulence factor regulatory genes, agr and sar, affect autolysis, resulting in decreased and increased autolysis rates, respectively. Zymographic analyses of these mutant strains suggest that agr and sar exert their effects on autolysis, in part, by modulating murein hydrolase expression and/or activity.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Bacterial Proteins / physiology*
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Bacteriolysis / drug effects*
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Excipients
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GTP-Binding Proteins / physiology*
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Monomeric GTP-Binding Proteins*
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Octoxynol
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Penicillins
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Peptidoglycan / biosynthesis
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Peptidoglycan / metabolism
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Staphylococcus aureus / drug effects*
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Staphylococcus aureus / pathogenicity
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Trans-Activators*
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Transcription Factors / physiology*
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Virulence
Substances
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Agr protein, Staphylococcus aureus
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Bacterial Proteins
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Excipients
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Penicillins
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Peptidoglycan
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SAR protein, Lycopersicon esculentum
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Trans-Activators
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Transcription Factors
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Octoxynol
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GTP-Binding Proteins
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Monomeric GTP-Binding Proteins