Independent ligand-induced folding of the RNA-binding domain and two functionally distinct antitermination regions in the phage lambda N protein

J Mogridge; P Legault; J Li; M D Van Oene; L E Kay; J Greenblatt

doi:10.1016/s1097-2765(00)80027-1

Independent ligand-induced folding of the RNA-binding domain and two functionally distinct antitermination regions in the phage lambda N protein

Mol Cell. 1998 Jan;1(2):265-75. doi: 10.1016/s1097-2765(00)80027-1.

Authors

J Mogridge¹, P Legault, J Li, M D Van Oene, L E Kay, J Greenblatt

Affiliation

¹ Banting and Best Department of Medical Research, Department of Molecular and Medical Genetics, University of Toronto, Ontario, Canada.

PMID: 9659923
DOI: 10.1016/s1097-2765(00)80027-1

Abstract

The transcriptional antitermination protein N of bacteriophage lambda binds the boxB component of the RNA enhancer nut (boxA + boxB) and the E. coli elongation factor NusA. Efficient antitermination by N requires an RNA-binding domain (amino acids 1-22) and two activating regions for antitermination: a newly identified NusA-binding region (amino acids 34-47) that suppresses NusA's enhancement of termination, and a carboxy-terminal region (amino acids 73-107) that interacts directly with RNA polymerase. Heteronuclear magnetic resonance experiments demonstrate that N is a disordered protein. Interaction with boxB RNA induces only the RNA-binding domain of N to adopt a folded conformation, while the activating regions of the protein remain disordered in the absence of their target proteins.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Bacterial Proteins / chemistry
Bacterial Proteins / metabolism
Bacteriophage lambda / chemistry*
Bacteriophage lambda / enzymology
Bacteriophage lambda / genetics*
Binding Sites / physiology
DNA-Directed RNA Polymerases / metabolism
Enhancer Elements, Genetic / physiology
Escherichia coli Proteins
Gene Expression Regulation, Viral*
Ligands
Magnetic Resonance Spectroscopy
Molecular Sequence Data
Peptide Elongation Factors*
Peptide Fragments / metabolism
Protein Structure, Tertiary
RNA, Viral / chemistry
RNA, Viral / genetics
RNA, Viral / metabolism
Regulatory Sequences, Nucleic Acid
Transcription Factors / chemistry
Transcription Factors / metabolism
Transcriptional Elongation Factors
Viral Regulatory and Accessory Proteins / chemistry*
Viral Regulatory and Accessory Proteins / genetics
Viral Regulatory and Accessory Proteins / metabolism*

Substances

Bacterial Proteins
Escherichia coli Proteins
Ligands
N protein, Bacteriophage lambda
Peptide Elongation Factors
Peptide Fragments
RNA, Viral
Transcription Factors
Transcriptional Elongation Factors
Viral Regulatory and Accessory Proteins
nusA protein, E coli
DNA-Directed RNA Polymerases