Abstract
The galE gene product, UDP-galactose 4-epimerase, mediates the incorporation of galactose in extracellular polysaccharide materials such as the O-side chain of lipopolysaccharide (LPS). The O-side chain in H. pylori LPS has been shown to cross-react with Lewis x and/or y blood group antigens, suggesting its potential involvement in H. pylori-linked autoimmune disease. To study its role in H. pylori LPS biosynthesis, the galE gene was cloned, sequenced, and a galE-knockout H. pylori strain was constructed. The H. pylori galE gene encoded a protein of 344 amino acids with a molecular weight of 39K. The LPS profile from the galE-knockout H. pylori strain showed a lower molecular weight than that of the parental strain, indicating the involvement of the galE gene in LPS biosynthesis of H. pylori.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Cloning, Molecular
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DNA, Bacterial / analysis
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DNA, Bacterial / genetics
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Electrophoresis, Polyacrylamide Gel
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Gene Expression Regulation / physiology
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Genes, Bacterial / genetics*
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Genomic Library
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Helicobacter pylori / chemistry
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Helicobacter pylori / genetics*
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Helicobacter pylori / metabolism*
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Lipopolysaccharides / analysis
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Lipopolysaccharides / biosynthesis*
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Molecular Sequence Data
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Mutagenesis, Insertional / genetics
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Mutagenesis, Insertional / physiology
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Mutation / genetics
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Mutation / physiology
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Sequence Analysis, DNA
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Sodium Dodecyl Sulfate
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UDPglucose 4-Epimerase / genetics*
Substances
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DNA, Bacterial
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Lipopolysaccharides
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Sodium Dodecyl Sulfate
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UDPglucose 4-Epimerase
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galactose epimerase