Abstract
The leukocyte-specific adapter molecule SLP-76 (Src homology 2 domain-containing leukocyte protein of 76 kilodaltons) is rapidly phosphorylated on tyrosine residues after receptor ligation in several hematopoietically derived cell types. Mice made deficient for SLP-76 expression contained no peripheral T cells as a result of an early block in thymopoiesis. Macrophage and natural killer cell compartments were intact in SLP-76-deficient mice, despite SLP-76 expression in these lineages in wild-type mice. Thus, the SLP-76 adapter protein is required for normal thymocyte development and plays a crucial role in translating signals mediated by pre-T cell receptors into distal biochemical events.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Adaptor Proteins, Signal Transducing
-
Animals
-
B-Lymphocytes / cytology
-
B-Lymphocytes / immunology
-
Gene Targeting
-
Immunoglobulin M / blood
-
Killer Cells, Natural / cytology
-
Leukopoiesis*
-
Lymph Nodes / cytology
-
Lymphocyte Activation
-
Lymphocyte Count
-
Macrophages / cytology
-
Mice
-
Mice, Inbred C57BL
-
Phosphoproteins / genetics
-
Phosphoproteins / physiology*
-
Protein-Tyrosine Kinases / metabolism
-
Receptors, Antigen, T-Cell / metabolism
-
Signal Transduction
-
Spleen / cytology
-
T-Lymphocytes / cytology*
-
Thymus Gland / cytology
-
ZAP-70 Protein-Tyrosine Kinase
Substances
-
Adaptor Proteins, Signal Transducing
-
Immunoglobulin M
-
Phosphoproteins
-
Receptors, Antigen, T-Cell
-
SLP-76 signal Transducing adaptor proteins
-
Protein-Tyrosine Kinases
-
ZAP-70 Protein-Tyrosine Kinase
-
Zap70 protein, mouse