HLA-A towards a high-resolution DNA typing

Tissue Antigens. 1998 May;51(5):549-52. doi: 10.1111/j.1399-0039.1998.tb02990.x.

Abstract

Sequencing-based typing (SBT) and sequence-specific oligonucleotide probing (PCR-SSOP) are DNA-based typing approaches to identify HLA-A alleles. In this study PCR-SSOP SBT have been evaluated and considered to reach a high-resolution typing. Based upon serological typing, 32 genomic samples were typed by SBT and PCR-SSOP Three main clusters of resolution could be defined. The advantage of the PCR-SSOP approach is the possibility to type numerous samples in a short time. SBT minimizes the number of ambiguous heterozygous combinations and often allows direct detection and identification of new alleles.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA
  • HLA-A Antigens / classification*
  • HLA-A Antigens / genetics*
  • Histocompatibility Testing
  • Humans
  • Polymerase Chain Reaction / methods*

Substances

  • HLA-A Antigens
  • DNA