Continuous wave excitation two-photon fluorescence microscopy exemplified with the 647-nm ArKr laser line

J Microsc. 1998 Jun;190(Pt 3):298-304. doi: 10.1046/j.1365-2818.1998.00375.x.

Abstract

We report on efficient two-photon fluorescence imaging in beam scanning microscopy by exciting UV dyes at the 647-nm line of a continuous wave ArKr mixed gas laser. For a numerical aperture of 1.4 (oil), we used an illumination power of up to 210 mW at the sample. High-resolution images were obtained for DAPI-labelled cell nuclei within 4-60 s. Our method is a simple two-photon alternative to UV confocal imaging with the potential of becoming a very useful feature of laser scanning microscopy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • DNA / analysis
  • Drosophila
  • Fibroblasts
  • Indoles
  • Lasers
  • Mice
  • Microscopy, Confocal / instrumentation
  • Microscopy, Fluorescence* / instrumentation
  • Photons

Substances

  • Indoles
  • DAPI
  • DNA