Histone-like TAFs within the PCAF histone acetylase complex

Cell. 1998 Jul 10;94(1):35-44. doi: 10.1016/s0092-8674(00)81219-2.

Abstract

PCAF histone acetylase plays a role in regulation of transcription, cell cycle progression, and differentiation. Here, we show that PCAF is found in a complex consisting of more than 20 distinct polypeptides. Strikingly, some polypeptides are identical to TBP-associated factors (TAFs), which are subunits of TFIID. Like TFIID, histone fold-containing factors are present within the PCAF complex. The histone H3- and H2B-like subunits within the PCAF complex are identical to those within TFIID, namely, hTAF(II)31 and hTAF(II)20/15, respectively. The PCAF complex has a novel histone H4-like subunit with similarity to hTAF(II)80 that interacts with the histone H3-like domain of hTAF(II)31. Moreover, the PCAF complex has a novel subunit with WD40 repeats having a similarity to hTAF(II)100.

Publication types

  • Comparative Study

MeSH terms

  • Acetyltransferases / chemistry*
  • Amino Acid Sequence
  • DNA-Binding Proteins / isolation & purification*
  • Fungal Proteins
  • HeLa Cells
  • Histone Acetyltransferases
  • Histones / isolation & purification*
  • Humans
  • Macromolecular Substances
  • Mass Spectrometry
  • Molecular Sequence Data
  • Nuclear Proteins / isolation & purification*
  • Protein Kinases
  • Saccharomyces cerevisiae Proteins*
  • Sequence Analysis
  • Sequence Homology, Amino Acid
  • Transcription Factors / isolation & purification*

Substances

  • DNA-Binding Proteins
  • Fungal Proteins
  • Histones
  • Macromolecular Substances
  • NGG1 protein, S cerevisiae
  • Nuclear Proteins
  • SPT3 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • Acetyltransferases
  • Histone Acetyltransferases
  • Protein Kinases

Associated data

  • GENBANK/AF069732
  • GENBANK/AF069733
  • GENBANK/AF069734
  • GENBANK/AF069735
  • GENBANK/AF069736