Abstract
c-Myc overexpression is associated with the locus-specific amplification and rearrangement of the dihydrofolate reductase (DHFR) gene. This has been shown in lymphoid and nonlymphoid cell lines. Furthermore, c-Myc-dependent DHFR gene amplification occurs independent of species origins; it has been described in rat, hamster, mouse, and human cell lines. Here, we report on c-Myc-dependent amplification of the DHFR gene in vivo, using an animal model of c-Myc-dependent neoplasia, the mouse plasmacytoma.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Chromosomes, Human, Pair 13 / genetics
-
Disease Models, Animal
-
Gene Amplification
-
Humans
-
Immunohistochemistry
-
In Situ Hybridization, Fluorescence
-
Mice
-
Mice, Inbred BALB C
-
Peritoneal Neoplasms / genetics
-
Peritoneal Neoplasms / metabolism
-
Plasmacytoma / genetics
-
Plasmacytoma / metabolism
-
Proto-Oncogene Proteins c-myc / genetics
-
Proto-Oncogene Proteins c-myc / metabolism*
-
Tetrahydrofolate Dehydrogenase / genetics*
Substances
-
Proto-Oncogene Proteins c-myc
-
Tetrahydrofolate Dehydrogenase