Abstract
N-acylhomoserine lactone (AHSL, autoinducer) is capable of regulating a set of genes by sensing cell density and developing an intercellular communication in Pseudomonas aeruginosa. Addition of AHSL in the exponential growth phase, regardless of cell density, induces a repression of cell growth of P. aeruginosa, an expression of stationary phase specific factor sigma 8 in vivo and a morphological change into smaller spherical shape indistinguishable from that in the stationary phase. It is demonstrated that AHSL can trigger an entry of bacteria into stationary phase as a growth controlling signal.
MeSH terms
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4-Butyrolactone / analogs & derivatives*
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4-Butyrolactone / pharmacology
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Bacterial Proteins / drug effects*
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Bacterial Proteins / metabolism
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Electrophoresis, Polyacrylamide Gel
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Homoserine / analogs & derivatives*
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Homoserine / pharmacology
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Immunoblotting
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Microscopy, Atomic Force
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Polymerase Chain Reaction
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Pseudomonas aeruginosa / drug effects*
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Pseudomonas aeruginosa / growth & development
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Pseudomonas aeruginosa / ultrastructure
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RNA, Bacterial / analysis
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RNA, Bacterial / isolation & purification
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RNA, Messenger / analysis
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RNA, Messenger / isolation & purification
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Sigma Factor / drug effects*
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Sigma Factor / metabolism
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Time Factors
Substances
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Bacterial Proteins
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N-butyrylhomoserine lactone
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RNA, Bacterial
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RNA, Messenger
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Sigma Factor
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sigma factor KatF protein, Bacteria
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Homoserine
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4-Butyrolactone