Microplate-array diagonal-gel electrophoresis (MADGE) and melt-MADGE: tools for molecular-genetic epidemiology

Trends Biotechnol. 1998 Jul;16(7):287-90. doi: 10.1016/s0167-7799(98)01217-7.

Abstract

Microplate-array diagonal-gel electrophoresis (MADGE) was invented for molecular-genetic epidemiological studies. It combines direct compatibility with microplates, convenient polyacrylamide-gel electrophoresis and economy of time and reagents at minimal capital cost, and enables one user to run up to several-thousand gel lanes per day for the direct assay of single-base variations. Melt-MADGE adds temporal-thermal-ramp apparatus to achieve similar throughput for de novo mutation scanning.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Electrophoresis / methods*
  • Molecular Biology*
  • Polymerase Chain Reaction