Abstract
Aplysia S-type K+ channels of sensory neurons play a dominant role in presynaptic facilitation and behavioural sensitization. They are closed by serotonin via cAMP-dependent phosphorylation, whereas they are opened by arachidonic acid, volatile general anaesthetics and mechanical stimulation. We have identified a cloned mammalian two P domain K+ channel sharing the properties of the S channel. In addition, the recombinant channel is opened by lipid bilayer amphipathic crenators, while it is closed by cup-formers. The cytoplasmic C-terminus contains a charged region critical for chemical and mechanical activation, as well as a phosphorylation site required for cAMP inhibition.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Aplysia
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Arachidonic Acid / pharmacology
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COS Cells
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Cyclic AMP-Dependent Protein Kinases / metabolism
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Humans
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Invertebrate Hormones / chemistry
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Invertebrate Hormones / physiology
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Ion Channel Gating / drug effects
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Ion Channel Gating / physiology*
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Molecular Sequence Data
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Phosphorylation
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Potassium Channels / chemistry*
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Potassium Channels / drug effects
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Potassium Channels / metabolism
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Potassium Channels / physiology*
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Potassium Channels, Tandem Pore Domain*
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Protein Structure, Tertiary
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Shab Potassium Channels
Substances
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Invertebrate Hormones
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KCNK1 protein, human
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Potassium Channels
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Potassium Channels, Tandem Pore Domain
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Shab Potassium Channels
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Shab protein, Aplysia
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potassium channel protein TREK-1
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Arachidonic Acid
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Cyclic AMP-Dependent Protein Kinases