Abstract
We report here the role of the CXC chemokine, epithelial neutrophil activating peptide (ENA-78), as an angiogenic factor in human non-small cell lung cancer (NSCLC). In freshly isolated human specimens of NSCLC, elevated levels of ENA-78 were found that strongly correlated with the vascularity of the tumors. In a SCID mouse model of human NSCLC tumorigenesis, expression of ENA-78 in developing tumors correlated with tumor growth in two different NSCLC cell lines. Furthermore, passive immunization of NSCLC tumor-bearing mice with neutralizing anti-ENA-78 antibodies reduced tumor growth, tumor vascularity, and spontaneous metastases, while having no effect on the proliferation of NSCLC cells either in vitro or in vivo. These findings suggest that ENA-78 is an important angiogenic factor in human NSCLC.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenocarcinoma / blood supply*
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Adenocarcinoma / metabolism
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Adenocarcinoma / pathology
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Animals
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Apoptosis
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Carcinoma, Non-Small-Cell Lung / blood supply*
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Carcinoma, Non-Small-Cell Lung / metabolism
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Carcinoma, Non-Small-Cell Lung / pathology
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Carcinoma, Squamous Cell / pathology
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Cell Division
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Chemokine CXCL5
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Chemokines, CXC*
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Female
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Humans
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Immunization, Passive
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Interleukin-8 / analogs & derivatives*
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Interleukin-8 / metabolism
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Interleukin-8 / physiology
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Lung Neoplasms / blood supply*
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Lung Neoplasms / metabolism
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Lung Neoplasms / pathology
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Mice
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Mice, SCID
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Neoplasm Proteins / physiology*
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Neoplasm Transplantation
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Neovascularization, Pathologic / physiopathology*
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Rats
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Tumor Cells, Cultured
Substances
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CXCL5 protein, human
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Chemokine CXCL5
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Chemokines, CXC
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Interleukin-8
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Neoplasm Proteins