Biochemical and molecular characterization of Leishmania parasites isolated from an endemic focus in eastern Sudan

L Oskam; F Pratlong; E E Zijlstra; C C Kroon; I P Dedet; P A Kager; G Schönian; H W Ghalib; A M el-Hassan; S E Meredith

doi:10.1016/s0035-9203(98)90982-8

Biochemical and molecular characterization of Leishmania parasites isolated from an endemic focus in eastern Sudan

Trans R Soc Trop Med Hyg. 1998 Jan-Feb;92(1):120-2. doi: 10.1016/s0035-9203(98)90982-8.

Authors

L Oskam¹, F Pratlong, E E Zijlstra, C C Kroon, I P Dedet, P A Kager, G Schönian, H W Ghalib, A M el-Hassan, S E Meredith

Affiliation

¹ Department of Biomedical Research, Royal Tropical Institute, Amsterdam, The Netherlands.

PMID: 9692173
DOI: 10.1016/s0035-9203(98)90982-8

Abstract

Twelve Leishmania isolates from visceral leishmaniasis patients in eastern Sudan were characterized using isoenzyme analysis, Southern blotting and polymerase chain reaction (PCR) 'fingerprinting'. Isoenzyme analysis revealed the presence of 3 zymodemes: MON-18, MON-30 and MON-82, corresponding to Leishmania donovani sensu stricto, L. infantum and L. archibaldi (still of uncertain taxonomic status), respectively. Southern blotting and PCR 'fingerprinting' revealed identical patterns for all 3 zymodemes, which were indistinguishable from those of L. donovani s.s.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Blotting, Southern
DNA, Protozoan / analysis
Humans
Isoenzymes / analysis
Leishmania donovani / isolation & purification*
Leishmania infantum / isolation & purification*
Leishmaniasis, Visceral / parasitology*
Longitudinal Studies
Polymerase Chain Reaction
Sudan

Substances

DNA, Protozoan
Isoenzymes