Copurification of P6, MRP8, and MRP14 from human granulocytes and separation of individual proteins

Protein Expr Purif. 1998 Aug;13(3):313-8. doi: 10.1006/prep.1998.0917.

Abstract

A method is described for purification of P6, MRP8, and MRP14, three calcium-binding proteins assigned to the S100 protein family. The purification procedure included preparation of human granulocytes, ammonium sulfate precipitation, and anion-exchange chromatography and resulted in the copurification of P6, MRP8, and MRP14. Individual proteins were separated by either preparative isoelectric focusing or preparative SDS-PAGE. The procedure was carried out in the course of 4 days and yielded several milligrams of essentially pure P6, MRP8, and MRP14 in either native or denatured form.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Antigens, Differentiation / blood
  • Antigens, Differentiation / chemistry
  • Antigens, Differentiation / isolation & purification*
  • Calcium-Binding Proteins / blood
  • Calcium-Binding Proteins / chemistry
  • Calcium-Binding Proteins / isolation & purification*
  • Calgranulin A
  • Calgranulin B
  • Cells, Cultured
  • Chromatography, Ion Exchange
  • Electrophoresis, Polyacrylamide Gel
  • Granulocytes / metabolism*
  • Humans
  • Isoelectric Focusing
  • Molecular Sequence Data
  • S100 Proteins*

Substances

  • Antigens, Differentiation
  • Calcium-Binding Proteins
  • Calgranulin A
  • Calgranulin B
  • S100 Proteins
  • calcium-binding protein p6, human