Summary report on the ISOBM TD-6 workshop: analysis of 20 monoclonal antibodies against Sialyl Lewisa and related antigens. Montreux, Switzerland, September 19-24, 1997

Tumour Biol. 1998;19(5):390-420. doi: 10.1159/000030032.

Abstract

The ISOBM TD-6 Workshop is the first international workshop on monoclonal antibodies against the Sialyl Lewisa (SLea) antigen. Eight research groups participated in a blind study to characterize the epitope binding, relative affinity and performance in immunoradiometric assays, of a panel of 20 monoclonal antibodies. The antibodies were tested against a diverse panel of neoglycoconjugates, purified antigens and human serum pools from gastrointestinal malignancies. Epitope specificities were determined for the majority of antibodies in the panel. Cross-reactivity with related saccharide structures was noted in several antibodies. Overall, the results of the TD-6 Workshop show further development of SLea immunoassays may yield yet more specific assays for the detection and management of gastrointestinal and other malignancies.

MeSH terms

  • Antibodies, Monoclonal* / immunology
  • Antibodies, Monoclonal* / metabolism
  • Antibody Specificity
  • Biomarkers, Tumor / analysis*
  • CA-19-9 Antigen / immunology
  • CA-19-9 Antigen / metabolism
  • Carbohydrate Sequence
  • Epitopes / immunology
  • Gangliosides / analysis*
  • Gangliosides / immunology
  • Gangliosides / metabolism
  • Gastrointestinal Neoplasms / blood
  • Humans
  • Immunoradiometric Assay
  • Molecular Sequence Data

Substances

  • Antibodies, Monoclonal
  • Biomarkers, Tumor
  • CA-19-9 Antigen
  • Epitopes
  • Gangliosides
  • sialyl Le(a) ganglioside