Development of specific nested oligonucleotide PCR primers for the Streptococcus iniae 16S-23S ribosomal DNA intergenic spacer

B R Berridge; J D Fuller; J de Azavedo; D E Low; H Bercovier; P F Frelier

doi:10.1128/JCM.36.9.2778-2781.1998

Development of specific nested oligonucleotide PCR primers for the Streptococcus iniae 16S-23S ribosomal DNA intergenic spacer

J Clin Microbiol. 1998 Sep;36(9):2778-81. doi: 10.1128/JCM.36.9.2778-2781.1998.

Authors

B R Berridge¹, J D Fuller, J de Azavedo, D E Low, H Bercovier, P F Frelier

Affiliation

¹ Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station, Texas 77843, USA. [email protected]

Abstract

Streptococcus iniae is a cause of septicemia, meningoencephalitis, and death in farmed fish and of cellulitis in human beings. A set of nested oligonucleotide PCR primers that specifically amplified a 373-bp subunit from a variety of clinical isolates from farmed fish and human patients were constructed from a 524-bp consensus sequence of the S. iniae 16S-23S ribosomal DNA intergenic spacer.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Animals, Domestic
Bacteremia / microbiology
Bacteremia / mortality
Bacteremia / veterinary
DNA Primers
DNA, Ribosomal / genetics*
Fish Diseases / diagnosis*
Fish Diseases / microbiology
Fish Diseases / mortality
Fishes / microbiology*
Humans
Meningoencephalitis / microbiology
Meningoencephalitis / mortality
Meningoencephalitis / veterinary
Polymerase Chain Reaction / methods*
RNA, Ribosomal, 16S / genetics
RNA, Ribosomal, 23S / genetics
Streptococcal Infections / diagnosis*
Streptococcal Infections / mortality
Streptococcal Infections / veterinary*
Streptococcus / classification
Streptococcus / genetics*
Streptococcus / isolation & purification*

Substances

DNA Primers
DNA, Ribosomal
RNA, Ribosomal, 16S
RNA, Ribosomal, 23S