Abstract
To clarify the reasons for the high fidelity of repetitive telomeric sequences, a series of d(TTXGGG)4 (X: A, G, C, and T) were synthesized and characterized by UV absorption, CD, chemical modification, and resistance to nucleases. d(TTCGGG)4, which is lacking in nature, has similar structural stability and resistance to nucleases, compared with d(TTAGGG)4, which is widely present at the telomeres of many organisms. d(TTGGGG)4 is the most stable as a result of formation of the four G-quartet layers in the presence of potassium ion.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Circular Dichroism
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Conserved Sequence / genetics
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DNA Methylation
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Electrophoresis, Polyacrylamide Gel
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Hydroxyl Radical / metabolism
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Kinetics
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Nucleic Acid Conformation
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Nucleic Acid Denaturation
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Oligodeoxyribonucleotides / chemistry*
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Repetitive Sequences, Nucleic Acid / genetics*
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Single-Strand Specific DNA and RNA Endonucleases / metabolism
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Spectrophotometry
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Telomere / genetics*
Substances
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Oligodeoxyribonucleotides
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Hydroxyl Radical
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Single-Strand Specific DNA and RNA Endonucleases