Abstract
Antimycin A and 2-heptyl-4-hydroxyquinoline N-oxide (HQNO), both of which bind to the same site of complex III, prevented U937 cell killing promoted by tert-butylhydroperoxide (tB-OOH). This cytoprotection was not directly caused by inhibition of electron transport or reduced formation of tB-OOH-derived toxic species, but rather appeared to be the consequence of a mechanism involving mitochondrial formation of hydrogen peroxide. Ubisemiquinone was most likely the electron donor allowing the formation of superoxides and, as a consequence, of hydrogen peroxide.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Bacterial Agents / pharmacology*
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Antimycin A / pharmacology*
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Apoptosis / drug effects*
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Cytochrome P-450 Enzyme Inhibitors
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Drug Interactions
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Electron Transport Complex III / antagonists & inhibitors
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Humans
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Hydrogen Peroxide / metabolism*
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Hydroxyquinolines / pharmacology
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Mitochondria / drug effects
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Mitochondria / metabolism*
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Peroxides / antagonists & inhibitors
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Peroxides / pharmacology*
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Tumor Cells, Cultured
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tert-Butylhydroperoxide
Substances
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Anti-Bacterial Agents
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Cytochrome P-450 Enzyme Inhibitors
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Hydroxyquinolines
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Peroxides
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2-(n-heptyl)-4-hydroxyquinoline N-oxide
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Antimycin A
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tert-Butylhydroperoxide
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Hydrogen Peroxide
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Electron Transport Complex III