High-level expression of mouse Mdr3 P-glycoprotein in yeast Pichia pastoris and characterization of ATPase activity

Methods Enzymol. 1998:292:397-413. doi: 10.1016/s0076-6879(98)92031-6.
No abstract available

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP Binding Cassette Transporter, Subfamily B*
  • ATP Binding Cassette Transporter, Subfamily B, Member 1 / biosynthesis*
  • ATP Binding Cassette Transporter, Subfamily B, Member 1 / isolation & purification
  • ATP Binding Cassette Transporter, Subfamily B, Member 1 / metabolism
  • ATP-Binding Cassette Transporters / biosynthesis*
  • ATP-Binding Cassette Transporters / isolation & purification
  • ATP-Binding Cassette Transporters / metabolism
  • Adenosine Triphosphatases / biosynthesis
  • Adenosine Triphosphatases / isolation & purification
  • Adenosine Triphosphatases / metabolism*
  • Affinity Labels / pharmacokinetics
  • Animals
  • Azides / pharmacokinetics
  • Binding, Competitive
  • Cell Fractionation / methods
  • Cell Membrane / metabolism
  • Cell Membrane / ultrastructure
  • Cloning, Molecular / methods
  • Colchicine / pharmacology
  • Doxorubicin / pharmacology
  • Drug Resistance, Multiple
  • Iodine Radioisotopes
  • Kinetics
  • Mice
  • Pichia* / genetics
  • Plasmids
  • Prazosin / analogs & derivatives
  • Prazosin / pharmacokinetics
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / isolation & purification
  • Recombinant Proteins / metabolism
  • Time Factors
  • Transformation, Genetic
  • Valinomycin / pharmacology
  • Vinblastine / pharmacology

Substances

  • ATP Binding Cassette Transporter, Subfamily B
  • ATP Binding Cassette Transporter, Subfamily B, Member 1
  • ATP-Binding Cassette Transporters
  • Affinity Labels
  • Azides
  • Iodine Radioisotopes
  • Recombinant Proteins
  • Valinomycin
  • Vinblastine
  • Doxorubicin
  • azidoprazosin
  • multidrug resistance protein 3
  • Adenosine Triphosphatases
  • Colchicine
  • Prazosin