HLA-G class I gene expression in normal and malignant hematopoietic cells

Hum Immunol. 1998 Aug;59(8):524-8. doi: 10.1016/s0198-8859(98)00041-x.

Abstract

The class Ib HLA-G gene encodes for a molecule which is selectively expressed in fetal placental cells. Fetomaternal tolerance could be partially explained by the interactions between HLA-G molecules and KIR receptors of decidual NK cells. To determine whether the presence of HLA-G antigens might constitute a factor of immune tolerance during the tumoral process, we compared the expression of the HLA-G gene in normal and malignant hematopoietic cells. Despite a HLA-G transcriptional activity in several lymphocytes and monocytes, no antigens are found at the cell surface or in the cytosol using the specific HLA-G mAb, 87G. This lack of expression does not appear modified in malignant hematopoietic cells. However, treatment of the monohistiocytic cell line U937 with different cytokines enabled the expression of HLA-G antigens to be induced. We suggest that the potential induction of HLA-G molecules in monocytic malignant cells following secretion of cytokines may constitute a factor of immune tolerance in patients.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line, Transformed / drug effects
  • Cytokines / pharmacology
  • DNA Primers / chemistry
  • Flow Cytometry
  • Gene Expression*
  • Genes, MHC Class I / genetics*
  • HLA Antigens / genetics*
  • HLA Antigens / metabolism
  • HLA-G Antigens
  • Histocompatibility Antigens Class I / genetics*
  • Histocompatibility Antigens Class I / metabolism
  • Humans
  • Immunoenzyme Techniques
  • Leukemia / genetics*
  • Leukemia / metabolism
  • Leukocytes / metabolism
  • Lymphoma, B-Cell / genetics*
  • Lymphoma, B-Cell / metabolism

Substances

  • Cytokines
  • DNA Primers
  • HLA Antigens
  • HLA-G Antigens
  • Histocompatibility Antigens Class I