Abstract
A comparative study of two techniques for the PCR genotyping of highly polymorphic tandem repeats was carried out by the example of a triplet repeat in the myotonin protein kinase gene. Sequencing denaturing gels were shown to yield more precise results in the analysis of amplification products.
MeSH terms
-
DNA, Satellite / genetics*
-
Electrophoresis, Polyacrylamide Gel
-
Genetic Markers*
-
Genotype
-
Myotonin-Protein Kinase
-
Polymerase Chain Reaction
-
Polymorphism, Genetic
-
Protein Kinases / genetics
-
Protein Serine-Threonine Kinases*
-
Repetitive Sequences, Nucleic Acid
Substances
-
DNA, Satellite
-
Genetic Markers
-
Protein Kinases
-
Myotonin-Protein Kinase
-
Protein Serine-Threonine Kinases