Abstract
In vitro transcription from the spoIIG promoter by Bacillus subtilis RNA polymerase reconstituted with wild-type alpha subunits and with C-terminal deletion mutants of the alpha subunit was equally stimulated by the response regulator Spo0A. Some differences in the structure of open complexes formed by RNA polymerase containing alpha subunit mutants were noted, although the wild-type and mutant polymerases appeared to use the same initiation mechanism.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Bacillus subtilis / genetics*
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Bacterial Proteins / metabolism*
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DNA-Directed RNA Polymerases / metabolism
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Promoter Regions, Genetic*
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Sigma Factor / genetics*
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Spores, Bacterial
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Transcription Factors / genetics*
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Transcription Factors / metabolism*
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Transcriptional Activation*
Substances
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Bacterial Proteins
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Sigma Factor
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Spo0A protein, Bacillus subtilis
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Transcription Factors
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sporulation-specific sigma factors
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DNA-Directed RNA Polymerases