Abstract
Elementary Ca2+ puffs form the basic building blocks of global Ins(1, 4,5)P3-evoked Ca2+ signals. In Xenopus oocytes, Ca2+ puffs evoked by the high-affinity agonist adenophostin were shorter and smaller than puffs evoked by Ins(1,4,5)P3 and the lower affinity analogue Ins(2,4, 5)P3. Agonist-specific mechanisms, therefore, play a role in shaping local Ca2+ release events, but termination of Ca2+ flux is not delimited simply by agonist dissociation.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenosine / analogs & derivatives
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Adenosine / pharmacology
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Animals
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Calcium Channels / chemistry*
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Calcium Signaling* / drug effects
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Cerebellum / metabolism
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Female
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In Vitro Techniques
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Inositol 1,4,5-Trisphosphate / metabolism*
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Inositol 1,4,5-Trisphosphate / pharmacology
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Inositol 1,4,5-Trisphosphate Receptors
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Inositol Phosphates / pharmacology
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Kinetics
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Microscopy, Confocal
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Oocytes / drug effects
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Oocytes / metabolism*
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Rats
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Receptors, Cytoplasmic and Nuclear / chemistry*
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Xenopus laevis
Substances
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Calcium Channels
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Inositol 1,4,5-Trisphosphate Receptors
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Inositol Phosphates
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Receptors, Cytoplasmic and Nuclear
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adenophostin A
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Inositol 1,4,5-Trisphosphate
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inositol 2,4,5-trisphosphate
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Adenosine