Characterisation of high Mr wheat glutenin polymers by agarose gel electrophoresis and dynamic light scattering

T A Egorov; T I Odintsova; P R Shewry; A S Tatham

doi:10.1016/s0014-5793(98)00983-1

Characterisation of high Mr wheat glutenin polymers by agarose gel electrophoresis and dynamic light scattering

FEBS Lett. 1998 Aug 28;434(1-2):215-7. doi: 10.1016/s0014-5793(98)00983-1.

Authors

T A Egorov¹, T I Odintsova, P R Shewry, A S Tatham

Affiliation

¹ Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow.

PMID: 9738481
DOI: 10.1016/s0014-5793(98)00983-1

Abstract

Agarose gel electrophoresis has been used to separate the complex mixture of wheat gluten polymers into fractions ranging in Mr, determined by dynamic light scattering, from about 500,000 to over 5x10(6). The separation is reliable and reproducible and well suited to the routine analysis of multiple samples.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Electrophoresis, Agar Gel
Glutens / analogs & derivatives*
Glutens / chemistry
Glutens / isolation & purification
Molecular Weight
Plant Proteins / chemistry
Plant Proteins / isolation & purification
Spectrum Analysis, Raman
Triticum / chemistry*

Substances

Plant Proteins
Glutens
glutenin