Abstract
We characterized two new members of the Zic family, Xenopus Zic1 and Zic2. They are very similar to mouse Zic1 and Zic2 in the protein coding region including the zinc finger domain. In early gastrula, Zic1 expression was restricted to the prospective neural plate region whereas Zic2 was expressed widely in the ectoderm. We observed enhanced neural and neural crest-derived tissue formation in the Zic1 or Zic2 overexpressed embryos and neural and neural crest marker induction in the Zic1 or Zic2 overexpressed animal cap explants. Our findings suggest that Zic1 and Zic2 have essentially the same properties as Zic3 and that the Xenopus Zic family may act cooperatively in the initial phase of neural and neural crest development.
Copyright 1998 Elsevier Science Ireland Ltd. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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DNA, Complementary / chemistry
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DNA, Complementary / genetics
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DNA, Complementary / isolation & purification
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Embryo, Nonmammalian / chemistry
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Embryo, Nonmammalian / metabolism
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Gene Expression Regulation, Developmental
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Genes / genetics
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In Situ Hybridization
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Molecular Sequence Data
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Nervous System / embryology*
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Neural Crest / embryology*
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Sequence Homology, Amino Acid
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Transcription Factors / genetics
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Transcription Factors / physiology*
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Xenopus Proteins*
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Xenopus laevis / embryology*
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Xenopus laevis / genetics
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Zinc Fingers / genetics
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Zinc Fingers / physiology*
Substances
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DNA, Complementary
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RNA, Messenger
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Transcription Factors
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Xenopus Proteins
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Zic1 protein, Xenopus
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Zic1 protein, mouse
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Zic2 protein, Xenopus
Associated data
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GENBANK/AB009564
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GENBANK/AB009565