Abstract
Two novel plasmid-mediated beta-lactamases (CTX-M-5 and CTX-M-6) produced by Salmonella typhimurium clinical strains were characterized. The enzymes exhibited a pI of 8.4, hydrolyzed oxyimino-beta-lactams and were susceptible to mechanism-based beta-lactamase inhibitors. The respective bla genes were cloned and sequenced. The deduced amino acid sequences showed a high degree of homology with those of the previously described plasmid class A CTX-M-type enzymes and appeared related to the chromosomal beta-lactamases of Klebsiella oxytoca.
MeSH terms
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Amino Acid Sequence
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Anti-Bacterial Agents / pharmacology
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Base Sequence
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Cefotaxime / metabolism*
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Cefotaxime / pharmacology
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Cephalosporin Resistance / genetics
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Cephalosporins / metabolism*
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Cephalosporins / pharmacology
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Genes, Bacterial
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Humans
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Microbial Sensitivity Tests
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Molecular Sequence Data
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Plasmids / genetics*
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Salmonella typhimurium / drug effects
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Salmonella typhimurium / enzymology
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Salmonella typhimurium / genetics*
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Sequence Analysis, DNA
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beta-Lactamases / chemistry
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beta-Lactamases / genetics*
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beta-Lactamases / metabolism*
Substances
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Anti-Bacterial Agents
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Cephalosporins
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beta-lactamase CTX-M-5
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beta-lactamase CTX-M-6
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beta-Lactamases
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Cefotaxime
Associated data
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GENBANK/AJ005044
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GENBANK/AJ005045