Abstract
A DNA fragment from Lactobacillus casei that restores growth to Escherichia coli and Salmonella typhimurium ptsH mutants on glucose and other substrates of the phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS) has been isolated. These mutants lack the HPr protein, a general component of the PTS. Sequencing of the cloned fragment revealed the absence of ptsH homologues. Instead, the complementation ability was located in a 120-bp fragment that contained a sequence homologue to the binding site of the Cra regulator from enteric bacteria. Experiments indicated that the reversion of the ptsH phenotype was due to a titration of the Cra protein, which allowed the constitutive expression of the fructose operon.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins / metabolism
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Cloning, Molecular
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DNA, Bacterial / genetics
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Escherichia coli / genetics*
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Escherichia coli Proteins*
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Fermentation
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Fructose / metabolism
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Genetic Complementation Test
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Lacticaseibacillus casei / genetics*
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Molecular Sequence Data
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Phosphoenolpyruvate Sugar Phosphotransferase System / genetics*
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Repressor Proteins / metabolism
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Salmonella typhimurium / genetics*
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
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Suppression, Genetic*
Substances
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Bacterial Proteins
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DNA, Bacterial
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Escherichia coli Proteins
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FruR protein, E coli
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Repressor Proteins
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FruR protein, Bacteria
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Fructose
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Phosphoenolpyruvate Sugar Phosphotransferase System
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phosphocarrier protein HPr