Abstract
We show the efficient transduction and expression of the lacZ gene in the skeletal muscle of adult C57BL/10ScSn mice after adenovirus-mediated gene transfer. Of the myofibers in the tibialis anterior muscle 62% were beta-galactosidase positive after injection of the lacZ gene under the control of the chicken beta-actin promoter and the cytomegalovirus enhancer. The transduced gene was preferentially expressed in type IIA and IIX fibers, which were richer in oxidative enzymes than type IIB fibers.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Actins / genetics
-
Adenoviridae / genetics*
-
Animals
-
Chickens
-
Enhancer Elements, Genetic / genetics
-
Gene Expression Regulation, Viral / genetics*
-
Immunohistochemistry
-
Mice
-
Mice, Inbred C57BL
-
Muscle Fibers, Skeletal / chemistry
-
Muscle Fibers, Skeletal / classification
-
Muscle Fibers, Skeletal / metabolism*
-
Muscle, Skeletal / cytology*
-
Muscle, Skeletal / metabolism*
-
Organ Specificity
-
Promoter Regions, Genetic
-
Transgenes / genetics*
-
beta-Galactosidase / analysis
-
beta-Galactosidase / biosynthesis
-
beta-Galactosidase / genetics
Substances
-
Actins
-
beta-Galactosidase