Fc-binding molecules specific for human IgG1 and IgG3 are present in Echinococcus granulosus protoscoleces

Parasite Immunol. 1998 Sep;20(9):399-404. doi: 10.1046/j.1365-3024.1998.00147.x.

Abstract

In this work we describe the presence of Fc-binding activity on the suckers and tegument of E. granulosus protoscoleces. A fraction (PSA-Fc+) from protoscolex somatic antigens was isolated by affinity chromatography on human Fc-gamma1-Sepharose. Analysis by SDS-PAGE of PSA-Fc+ showed that it contained two major components. Using mouse F(ab')2-human Fc chimaeric monoclonal antibodies we verified that PSA-Fc+ bound mainly to human Fc-gamma1 and Fc-gamma3 isotypes. In addition, one of the components of PSA-Fc+ showed proteolytic activity. Both, Fc-binding and proteolytic activities localized on the protoscolex surface, may play a relevant role in the host-parasite interaction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Helminth / metabolism*
  • Antibodies, Monoclonal
  • Antigens, Helminth / metabolism*
  • Echinococcus / growth & development
  • Echinococcus / immunology*
  • Echinococcus / pathogenicity
  • Host-Parasite Interactions / immunology
  • Humans
  • Immunoglobulin Fc Fragments / metabolism
  • Immunoglobulin G / metabolism*
  • Mice
  • Receptors, Fc / metabolism*

Substances

  • Antibodies, Helminth
  • Antibodies, Monoclonal
  • Antigens, Helminth
  • Immunoglobulin Fc Fragments
  • Immunoglobulin G
  • Receptors, Fc