Approaches to study interactions between small DNA viruses and differentiated tissue

Methods. 1998 Sep;16(1):62-82. doi: 10.1006/meth.1998.0645.

Abstract

Polyomavirus (Py) derives its name from the early observation of multiple tumors that develop in newborn mice following inoculation with this family of viruses. In nature, however, tumor development is rare in the virus life cycle, rather a two-phase infection occurs, acute and persistent, resulting in a final latent infection in the kidneys. The acute phase induces an antiviral immune response, although no recognizable inflammation, which can last the lifetime of the mouse, even passing on antibodies to its offspring. The structure, replication, and expression of the Py viral genome in permissive and nonpermissive infections has been studied extensively in various cell culture systems. However, the nature of Py expression, replication, and immunopathogenesis in mice has not been thoroughly researched.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cytokines / metabolism
  • DNA Viruses / immunology
  • DNA Viruses / metabolism
  • DNA, Viral / analysis
  • Fluorescent Antibody Technique
  • Gene Expression Regulation, Viral / genetics
  • Histocytochemistry
  • In Situ Hybridization
  • Injections / methods
  • Macrophages / virology
  • Mice
  • Mice, Inbred Strains
  • Neoplasms, Experimental
  • Polymerase Chain Reaction
  • Polyomavirus / immunology
  • Polyomavirus / metabolism*
  • Polyomavirus Infections / immunology
  • Polyomavirus Infections / virology*
  • Tissue Transplantation
  • Tumor Virus Infections / immunology
  • Tumor Virus Infections / virology*

Substances

  • Cytokines
  • DNA, Viral