Abstract
We describe functional binding sites for the tumor suppressor p53 and for NFkappaB residing in the promoter of the novel human early response gene p22/PRG1 (IEX-1/DIF-2). Gel shift and supershift assays demonstrate binding of p53 and NFkappaB to their corresponding sites in vitro. CAT-reporter gene assays show transactivation of the human p22/PRG1 promoter by p53 in Hep3B cells stably transfected with a temperature-sensitive mutant p53, but not in p53-deficient Hep3B cells. TNF alpha induced NFkappaB dependent transactivation was shown in HepG2 cells or in 818-4 pancreatic cancer cells. These data imply that human p22/PRG1 is a target gene for p53 and NFkappaB involved in growth regulation and stress response.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Apoptosis Regulatory Proteins
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Base Sequence
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Binding Sites
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Carcinoma, Hepatocellular
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Cell Division
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Chloramphenicol O-Acetyltransferase / biosynthesis
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Chloramphenicol O-Acetyltransferase / genetics
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Genes, Immediate-Early
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Glycoproteins / biosynthesis
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Glycoproteins / genetics*
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Humans
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Immediate-Early Proteins*
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Liver Neoplasms
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Membrane Proteins
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Molecular Sequence Data
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NF-kappa B / metabolism*
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Neoplasm Proteins*
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Phosphoproteins / biosynthesis
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Phosphoproteins / genetics*
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Promoter Regions, Genetic*
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Recombinant Fusion Proteins / biosynthesis
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Transcription, Genetic*
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Transfection
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Tumor Cells, Cultured
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Tumor Suppressor Protein p53 / metabolism*
Substances
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Apoptosis Regulatory Proteins
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Glycoproteins
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IER3 protein, human
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Immediate-Early Proteins
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Membrane Proteins
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NF-kappa B
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Neoplasm Proteins
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Phosphoproteins
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Recombinant Fusion Proteins
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Tumor Suppressor Protein p53
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Chloramphenicol O-Acetyltransferase