An interactive ion channel permeation tutorial was developed using a Microsoft Excel v5.0 spreadsheet to describe a two barrier, one site channel model by means of Eyring rate theory (ERT). The spreadsheet is inherently interactive so that the user receives immediate feedback about how changes in the energy barrier profile or ion concentrations affect current-voltage relations and single channel conductance. The spreadsheet model is easy to use, allows direct access to intermediate calculated values and all equations, contains graphical displays of parameters and lends itself to customization by a user having only a basic knowledge of spreadsheet operations.