Abstract
Of the 69 Helicobacter pylori isolates analysed, 31 strains (45%) showed resistance to metronidazole, one strain (1.4%) was resistant to amoxicillin while two strains (2.9%) were resistant to clarithromycin. It was found that metronidazole resistance rates increased in Singapore from 20% to 62% between late 1995 and early 1997. By biotyping using API ZYM, a total of 80% (55/69) strains were characterized as biotype II, while the remaining 20% (14/69) strains belonged to biotype III. Interestingly, 71% (10/14) of biotype III were resistant to metronidazole compared with 38% (21/55) of biotype II. DNA profiles generated by random amplified polymorphic DNA from 69 isolates showed highly diversified DNA fingerprints allowing effective discrimination among strains. Of the 60 H. pylori isolates from peptic ulcer patients, it was found that cagA and vacA occurred in 80% (48/60) and 82% (49/60) isolates, respectively.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amoxicillin / pharmacology
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Anti-Bacterial Agents / pharmacology
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Antigens, Bacterial*
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Bacterial Proteins / chemistry
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Bacterial Proteins / genetics
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Bacterial Typing Techniques
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Biopsy
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Clarithromycin / pharmacology
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Cytotoxins / chemistry
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Cytotoxins / genetics
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DNA Primers / chemistry
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DNA, Bacterial / chemistry
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Drug Resistance, Microbial
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Electrophoresis, Agar Gel
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Helicobacter Infections / epidemiology
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Helicobacter Infections / immunology
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Helicobacter Infections / microbiology*
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Helicobacter pylori / classification*
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Helicobacter pylori / drug effects
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Helicobacter pylori / immunology
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Humans
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Metronidazole / pharmacology
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Penicillins / pharmacology
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Peptic Ulcer / epidemiology
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Peptic Ulcer / immunology
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Peptic Ulcer / microbiology*
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Polymerase Chain Reaction
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Prevalence
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Random Amplified Polymorphic DNA Technique
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Singapore / epidemiology
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Stomach / microbiology
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Tetracycline Resistance
Substances
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Anti-Bacterial Agents
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Antigens, Bacterial
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Bacterial Proteins
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Cytotoxins
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DNA Primers
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DNA, Bacterial
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Penicillins
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VacA protein, Helicobacter pylori
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cagA protein, Helicobacter pylori
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Metronidazole
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Amoxicillin
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Clarithromycin