Abstract
A partial cDNA clone encoding the porcine proteasome subunit A4 (PSMA4 or proteasome subunit C9) has been isolated from a porcine muscle cDNA library and sequenced. A biallelic TaqI RFLP was identified in Large White, Landrace and Duroc breeds. Moreover, the 3'-untranslated region of the gene showed a triallelic SSCP. By linkage analysis the PSMA4 locus was assigned to pig chromosome 7 and by radioactive in situ hybridization this locus was mapped to the region 7q13-q14.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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3' Untranslated Regions
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Alleles
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Animals
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Base Sequence
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Cysteine Endopeptidases / chemistry
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Cysteine Endopeptidases / genetics*
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DNA, Complementary / genetics
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DNA, Complementary / isolation & purification
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Gene Frequency
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Genetic Linkage
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Humans
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In Situ Hybridization
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Molecular Sequence Data
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Multienzyme Complexes / chemistry
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Multienzyme Complexes / genetics*
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Physical Chromosome Mapping
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Polymorphism, Single-Stranded Conformational
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Proteasome Endopeptidase Complex
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Protein Conformation
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Rats
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Species Specificity
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Swine / genetics*
Substances
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3' Untranslated Regions
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DNA, Complementary
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Multienzyme Complexes
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Cysteine Endopeptidases
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Proteasome Endopeptidase Complex
Associated data
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GENBANK/X91847
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GENBANK/X91848