Abstract
Nitric oxide (NO) may modulate the catalytic activity of cysteine proteases. In the present study, the inhibitory effect of NO, released by the NO-donors (+/-)-(E)-4-ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide and nitroprusside, on papain action is reported. Papain inactivation via NO-mediated nitrosylation of the Cys25 catalytic residue represents a molecular model for cysteine protease inhibition.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cysteine / metabolism*
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Cysteine Proteinase Inhibitors / pharmacology*
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Nitric Oxide / metabolism
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Nitric Oxide / pharmacology*
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Nitro Compounds / pharmacology
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Nitroprusside / pharmacology
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Papain / antagonists & inhibitors*
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Papain / metabolism*
Substances
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Cysteine Proteinase Inhibitors
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Nitro Compounds
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Nitroprusside
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Nitric Oxide
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FK 409
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Papain
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Cysteine