Granulocyte-macrophage colony-stimulating factor (GM-CSF)-coated implants and their potential for reducing biomaterial-associated infection in neutropenic hosts

Zentralbl Bakteriol. 1998 Oct;288(2):237-51. doi: 10.1016/s0934-8840(98)80046-7.

Abstract

The incidence of infections associated with the use of medical biomaterials is high for skin-penetrating devices, when microbes of the normal skin flora like coagulase-positive and coagulase-negative staphylococci dominate as causative organisms. The most serious ones are infections in immunocompromised individuals. A mouse model of subcutaneous staphylococcal infection yielding abscesses in cyclophosphamide-induced neutropenic mice implanted with heparinized polyethylene (H-PE) was used. The present study addresses the question of the effects of implant modification with recombinant granulocyte-macrophage stimulating factor (rGM-CSF) on the course of infection. Our findings demonstrate that such modification reduces the proliferation of bacteria within the abscess and as a consequence limits the dissemination of bacteria from the local infection induced in the neutropenic host.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Abscess / microbiology
  • Abscess / prevention & control*
  • Animals
  • Anti-Bacterial Agents / pharmacology*
  • Colony Count, Microbial
  • Disease Models, Animal
  • Female
  • Granulocyte-Macrophage Colony-Stimulating Factor / pharmacology*
  • Mice
  • Mice, Inbred BALB C
  • Neutropenia / complications*
  • Neutrophils / immunology
  • Neutrophils / microbiology
  • Polyethylenes
  • Recombinant Proteins
  • Staphylococcal Infections / microbiology
  • Staphylococcal Infections / prevention & control*
  • Staphylococcus aureus / growth & development*
  • Staphylococcus epidermidis / growth & development*

Substances

  • Anti-Bacterial Agents
  • Polyethylenes
  • Recombinant Proteins
  • Granulocyte-Macrophage Colony-Stimulating Factor