Abstract
The meningococcal hemA gene was cloned and used to construct a porphyrin biosynthesis mutant. An analysis of the hemA mutant indicated that meningococci can transport intact porphyrin from heme (Hm), hemoglobin (Hb), and Hb-haptoglobin (Hp). By constructing a HemA- HpuAB- double mutant, we demonstrated that HpuAB is required for the transport of porphyrin from Hb and Hb-Hp.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Aldehyde Oxidoreductases / genetics
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Aldehyde Oxidoreductases / metabolism
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Bacterial Outer Membrane Proteins / genetics
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Bacterial Outer Membrane Proteins / metabolism*
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Base Sequence
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Biological Transport
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Carrier Proteins / metabolism*
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DNA, Bacterial
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Haptoglobins / metabolism*
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Hemoglobins / metabolism*
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Lipoproteins / genetics
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Lipoproteins / metabolism
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Molecular Sequence Data
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Mutagenesis
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Neisseria meningitidis / genetics
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Neisseria meningitidis / metabolism*
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Porphyrins / metabolism*
Substances
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Bacterial Outer Membrane Proteins
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Carrier Proteins
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DNA, Bacterial
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Haptoglobins
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Hemoglobins
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HpuB protein, Neisseria
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Lipoproteins
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Porphyrins
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Aldehyde Oxidoreductases
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glutamyl tRNA reductase
Associated data
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GENBANK/AF067426
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GENBANK/AF067427