Abstract
The gene encoding glucose 6-phosphate dehydrogenase (G6PD), which plays a pivotal role in cell defense against oxidative stress, is ubiquitously expressed at widely different levels in various tissues; moreover, G6PD expression is regulated by a number of stimuli. In this study we have analyzed the molecular anatomy of the G6PD core promoter. Our results indicate that the G6PD promoter is more complex than previously assumed; G6PD expression is under the control of several elements that are all required for correct promoter functioning and, furthermore, a still unidentified mammalian specific factor is needed.
MeSH terms
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Animals
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Base Sequence
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Binding Sites / genetics
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Cell Line
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DNA-Binding Proteins / metabolism
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DNA-Binding Proteins / physiology
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Drosophila / genetics
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Gene Expression Regulation
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Glucosephosphate Dehydrogenase / genetics*
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Humans
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Molecular Sequence Data
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Mutagenesis, Site-Directed
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Nucleic Acid Conformation
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Promoter Regions, Genetic / genetics*
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Sp1 Transcription Factor / metabolism
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Sp1 Transcription Factor / physiology
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Sp3 Transcription Factor
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Transcription Factors / metabolism
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Transcription Factors / physiology
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Transcriptional Activation
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Zinc Fingers
Substances
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DNA-Binding Proteins
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Sp1 Transcription Factor
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Transcription Factors
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Sp3 Transcription Factor
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Glucosephosphate Dehydrogenase