Abstract
Our laboratory recently recovered Chlamydia trachomatis in tissue culture from a urogenital specimen which tested negative by commercial plasmid-based PCR. Immunotyping and omp1 sequencing identified the isolate as a serovar E isolate. Further investigation by PCR and Southern hybridization indicated that this isolate lacks the chlamydial cryptic plasmid.
Publication types
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Case Reports
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Bacterial Outer Membrane Proteins
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Bacterial Typing Techniques
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Chlamydia Infections / diagnosis*
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Chlamydia trachomatis / classification
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Chlamydia trachomatis / genetics
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Chlamydia trachomatis / isolation & purification*
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False Negative Reactions
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Humans
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Male
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Male Urogenital Diseases / diagnosis*
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Plasmids*
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Polymerase Chain Reaction
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Reagent Kits, Diagnostic
Substances
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Bacterial Outer Membrane Proteins
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Reagent Kits, Diagnostic