Generation, identification, and evaluation of expressed sequence tags from different developmental stages of the Asian blood fluke Schistosoma japonicum

Biochem Biophys Res Commun. 1998 Nov 18;252(2):348-56. doi: 10.1006/bbrc.1998.9491.

Abstract

Here we report 658 expressed sequence tags (ESTs) generated from the 5'-termini of clones randomly selected from directional cDNA libraries constructed from mRNAs from three developmental stages of Schistosoma japonicum. Putative identifications were assigned to 46. 2% of the ESTs; 6.4% were previously known from S. japonicum, 5.6% were previously known from S. mansoni, 34.2% were known from other organisms, and the remaining 53.8% may represent S. japonicum-specific genes. These 658 ESTs appeared to be derived from 457 unique genes, which together represent 2 to 3% of the 15,000 to 20,000 genes predicted to occur in the schistosome genome.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • DNA Primers / genetics
  • DNA, Complementary / genetics
  • DNA, Helminth / genetics
  • Expressed Sequence Tags*
  • Female
  • Gene Expression Regulation, Developmental
  • Gene Library
  • Genes, Helminth
  • Male
  • Mice
  • RNA, Helminth / genetics
  • RNA, Messenger / genetics
  • Rabbits
  • Schistosoma japonicum / genetics*
  • Schistosoma japonicum / growth & development*

Substances

  • DNA Primers
  • DNA, Complementary
  • DNA, Helminth
  • RNA, Helminth
  • RNA, Messenger