Abstract
We investigated HSPs mRNA expression in cultured fibroblasts from control and AD patients. Northern blot analysis using probes for HSP70 and HSC73 revealed that HS induced a several fold increase in both mRNA. Under these condition the extent of mRNA increase was similar in controls and AD. HS elicited also a modest increase in sAPP release that was similar in control and AD. The results suggest that the ability of AD fibroblasts to produce a defensive response to HS is preserved.
MeSH terms
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Aged
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Alzheimer Disease / pathology
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Alzheimer Disease / physiopathology*
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Amyloid beta-Protein Precursor / genetics
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Carrier Proteins / genetics
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Cells, Cultured
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Female
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Fibroblasts / physiology*
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HSC70 Heat-Shock Proteins
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HSP70 Heat-Shock Proteins / genetics
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Hot Temperature*
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Humans
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Middle Aged
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RNA, Messenger / metabolism
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Reference Values
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Shock / pathology
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Shock / physiopathology*
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Solubility
Substances
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Amyloid beta-Protein Precursor
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Carrier Proteins
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HSC70 Heat-Shock Proteins
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HSP70 Heat-Shock Proteins
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HSPA8 protein, human
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RNA, Messenger