Abstract
The isolation and characterization of rabbit and human cDNAs revealed a new low density lipoprotein receptor (LDLR)-related protein (LRP) designated as LRP5. Human LRP5 cDNA encodes a 1, 616-amino acid type I membrane-like protein with three ligand binding repeats in its extracellular region. LDLR-deficient cells transduced by recombinant adenovirus containing human LRP5 exhibited increased binding of apolipoprotein E (apoE)-enriched beta-migrating very low density lipoprotein. Northern blotting and in situ hybridization revealed a high level of LRP5 expression in hepatocytes and the adrenal gland cortex. In LDLR-deficient Watanabe heritable hyperlipidemic rabbits, LRP5 mRNA was increased in the liver and accumulated in cholesterol-laden foam cells of atherosclerotic lesions.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adrenal Cortex / chemistry
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Adrenal Cortex / metabolism*
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Amino Acid Sequence
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Animals
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Apolipoproteins E / chemistry
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Apolipoproteins E / metabolism*
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Arteriosclerosis / metabolism
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Binding Sites
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Blotting, Northern
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Cholesterol / metabolism
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Cloning, Molecular
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Cytoplasm / metabolism
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Humans
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Hyperlipidemias / metabolism
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In Situ Hybridization / methods
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LDL-Receptor Related Proteins
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Liver / chemistry
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Liver / metabolism*
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Low Density Lipoprotein Receptor-Related Protein-5
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Male
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Molecular Sequence Data
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Rabbits
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Receptors, LDL / genetics*
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Receptors, LDL / metabolism*
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Transcription, Genetic
Substances
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Apolipoproteins E
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LDL-Receptor Related Proteins
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LRP5 protein, human
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Low Density Lipoprotein Receptor-Related Protein-5
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Receptors, LDL
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Cholesterol
Associated data
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GENBANK/AB017498
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GENBANK/AB017499