Isolation of translactone-containing triterpenes with thrombin inhibitory activities from the leaves of Lantana camara

M J O'Neill; J A Lewis; H M Noble; S Holland; C Mansat; J E Farthing; G Foster; D Noble; S J Lane; P J Sidebottom; S M Lynn; M V Hayes; C J Dix

doi:10.1021/np970464j

Isolation of translactone-containing triterpenes with thrombin inhibitory activities from the leaves of Lantana camara

J Nat Prod. 1998 Nov;61(11):1328-31. doi: 10.1021/np970464j.

Authors

M J O'Neill¹, J A Lewis, H M Noble, S Holland, C Mansat, J E Farthing, G Foster, D Noble, S J Lane, P J Sidebottom, S M Lynn, M V Hayes, C J Dix

Affiliation

¹ GlaxoWellcome Research and Development, Medicines Research Centre, Gunnels Wood Road, Stevenage, SG1 2NY, UK. [email protected]

PMID: 9834145
DOI: 10.1021/np970464j

Abstract

Methanolic extracts prepared from the leaves of Lantana camara have been found to inhibit human thrombin. An assay, in which thrombin activity is measured as a function of clot formation from fibrinogen, was used to guide the fractionation and purification of five principal active constituents (1-5), which were all characterized as 5,5-trans-fused cyclic lactone-containing euphane triterpenes.

MeSH terms

Carbohydrate Sequence
Crystallography, X-Ray
Fibrinogen / antagonists & inhibitors
Humans
Molecular Sequence Data
Plant Extracts / chemistry
Plant Leaves / chemistry*
Plants, Medicinal / chemistry*
Spectrophotometry, Infrared
Spectrophotometry, Ultraviolet
Thrombin / antagonists & inhibitors*
Triterpenes / isolation & purification
Triterpenes / pharmacology*

Substances

Plant Extracts
Triterpenes
Fibrinogen
Thrombin