The olivocochlear bundle (OCB) stems from the superior olivary complex in the brainstem and projects to the ipsilateral and contralateral cochlea. Several studies have suggested that the OCB has a suppressive effect on the inner ear by inhibiting the responses of the primary afferent fibers. To evaluate the action of OCB by more available measurement, radiofrequency lesion was applied to 30 Wistar rats which were divided into four groups, a sham group and another three groups with different OCB lesion sites. Consequent changes following OCB lesion were evaluated by measuring the brainstem auditory evoked potentials (BAEPs), which are considered to reflect the auditory brainstem afferent conduction. The amplitudes and latencies did not change significantly after sham treatment. However, although the BAEP peak latencies were not significantly altered after damaging the OCB, the amplitudes of the BAEP waves I to III were augmented significantly. These results indicate that increased neural activities were presented in the auditory nerves, cochlear nucleus and the superior olivary complex upon disinhibition of the OCB. Unilateral OCB lesions predominantly augmented BAEP waveform which was recorded ipsilaterally to the lesion side. Upon lesion of the midline OCB, BAEP recordings on either side showed significant increments in the amplitudes of waves I to III. These findings are compatible with those observed in the cat model and other rodents, and thus confirm that (1) OCB lesion leads to increased amplitudes in some BAEP peaks which were evoked by certain hyperactive auditory nuclei and tracts; and (2) BAEP measurment is a convenient and useful tool for assessing the OCB function.