Abstract
The subcellular localization of COP1, a key photomorphogenic repressor, is regulated by light in Arabidopsis seedlings. Photoreceptor loss-of-function mutants and dominant gain-of-function overexpression transgenes were both used to analyze the influences of the three photoreceptors, phyA, phyB, and CRY1, on the light-regulated subcellular localization of COP1. Through a semiquantitative analysis of the nuclear abundance of GUS-COP1 in the various genetic backgrounds, the specific roles of the individual photoreceptors have been established. The data suggest that multiple photoreceptors influence the light-regulated subcellular localization of COP1 in white light. Under specific wavelengths of light, phyA, phyB, and CRY1 each play critical roles in mediating far-red, red, and blue light signals, respectively. Our data also support an interdependency between CRY1 and the phytochromes in mediating the light-regulated subcellular localization of COP1 and thus seedling development.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Arabidopsis / metabolism*
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Arabidopsis / radiation effects
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Arabidopsis Proteins*
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Carrier Proteins / genetics
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Carrier Proteins / metabolism*
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Carrier Proteins / radiation effects
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Cell Nucleus / metabolism*
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Cell Nucleus / radiation effects
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Cryptochromes
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Drosophila Proteins*
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Eye Proteins*
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Flavoproteins / metabolism
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Genes, Reporter
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Glucuronidase / genetics
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Glucuronidase / metabolism
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Light
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Photoreceptor Cells*
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Photoreceptor Cells, Invertebrate*
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Photosynthetic Reaction Center Complex Proteins / metabolism*
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Photosynthetic Reaction Center Complex Proteins / radiation effects
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Phytochrome / metabolism
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Phytochrome A
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Phytochrome B
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Plant Proteins / genetics
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Plant Proteins / metabolism*
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Plant Proteins / radiation effects
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Receptors, G-Protein-Coupled
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Repressor Proteins / genetics
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Repressor Proteins / metabolism*
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Repressor Proteins / radiation effects
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Seeds / genetics
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Seeds / metabolism
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Seeds / radiation effects
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Transcription Factors*
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Transgenes
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Ubiquitin-Protein Ligases*
Substances
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Arabidopsis Proteins
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CRY1 protein, Arabidopsis
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Carrier Proteins
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Cryptochromes
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Drosophila Proteins
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Eye Proteins
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Flavoproteins
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PHYA protein, Arabidopsis
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PHYB protein, Arabidopsis
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Photosynthetic Reaction Center Complex Proteins
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Phytochrome A
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Plant Proteins
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Receptors, G-Protein-Coupled
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Recombinant Fusion Proteins
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Repressor Proteins
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Transcription Factors
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cry protein, Drosophila
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Phytochrome
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Phytochrome B
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AT2G32950 protein, Arabidopsis
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Ubiquitin-Protein Ligases
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Glucuronidase