In this paper we reported that the nucleocapsid morphogenetic process of AcMNPV in which polyhedrin gene was deleted in the nuclei of sf9 cells. First, the capsid proteins entered the nuclei and assembled many 34nm diameter long fascicularly arranged hollow-tube structures. Then viral DNA entered these tubes that turned into solid structure with high electronic density. The solid structure separated at a certain distance to form 34 x 26 nm fascicular bacilliform structure known as nucleocapsid. Finally, fascicular nucleocapsids were wrapped by envelope and became complete multicapsid morphotype viron.