Abstract
Trophozoites of virulent Entamoeba histolytica transfected with the antisense gene encoding cysteine proteinase 5 (CP5) have only 10% of the CP activity but retain their cytopathic activity on mammalian monolayers. In the present study we found that the transfected trophozoites with low levels of CP activity were incapable of inducing the formation of liver lesions in hamsters.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cricetinae
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Cysteine Endopeptidases / biosynthesis
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Cysteine Endopeptidases / genetics*
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Cysteine Proteinase Inhibitors / genetics*
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Cysteine Proteinase Inhibitors / physiology
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Entamoeba histolytica / enzymology*
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Entamoeba histolytica / genetics
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Entamoeba histolytica / pathogenicity
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Entamoebiasis / enzymology
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Entamoebiasis / parasitology*
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Entamoebiasis / pathology
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Gene Expression Regulation
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Liver Abscess, Amebic / enzymology
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Liver Abscess, Amebic / parasitology*
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Liver Abscess, Amebic / pathology
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Mesocricetus
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RNA, Antisense / physiology*
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Transfection
Substances
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Cysteine Proteinase Inhibitors
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RNA, Antisense
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Cysteine Endopeptidases