Penicillium species were isolated from 1073 adult mosquitoes and larvae of Aedes spp., Anopheles spp., Culex spp. and Mansonia spp. captured in the northern and southeastern regions of Brazil. In a total of 24 collections, 198 Penicillium strains were identified in 13 species: Penicillium canescens, P. chrysogenum, P. citrinum, P. corylophilum, P. decumbens, P. expansum, P. fellutanum, P. implicatum, P. janthinellum, P. oxalicum, P. purpurogenum, P. viridicatum and P. waksmanii. The total isolation frequency of the Penicillium species in the investigated mosquitoes was: Anopheles spp. (51.5%), Aedes spp. (15.1%), Culex spp. (23.7%) and Mansonia spp. (10.1%). The Penicillium species with the highest incidence in the mosquito genera were: P. corylophilum, P. janthinellum, P. fellutanum and P. waksmanii. The highest number of mosquitoes were captured in the state of Rio de Janeiro, followed by Rondônia and Minas Gerais.