In vitro selection of phosphorothiolated aptamers

Bioorg Med Chem Lett. 1998 Sep 8;8(17):2285-90. doi: 10.1016/s0960-894x(98)00414-4.

Abstract

A pool of RNA molecules that contained exclusively phosphorothioate internucleoside linkages was used as a starting point for the selection of aptamers that bind to basic fibroblast growth factor (bFGF), and appear to act as heparin mimics.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Binding Sites
  • Cloning, Molecular
  • DNA / chemistry*
  • DNA Primers / chemistry
  • Fibroblast Growth Factor 2 / genetics
  • Fibroblast Growth Factor 2 / metabolism*
  • Heparin / metabolism
  • Humans
  • Nucleic Acid Conformation*
  • Oligodeoxyribonucleotides / chemical synthesis*
  • Oligodeoxyribonucleotides / chemistry
  • Oligodeoxyribonucleotides / metabolism
  • RNA / chemical synthesis*
  • RNA / chemistry
  • RNA / metabolism
  • Recombinant Proteins / metabolism
  • Templates, Genetic
  • Thionucleotides*
  • Transcription, Genetic

Substances

  • DNA Primers
  • Oligodeoxyribonucleotides
  • Recombinant Proteins
  • Thionucleotides
  • Fibroblast Growth Factor 2
  • RNA
  • Heparin
  • DNA